Plasma membrane vesicles from cauliflower meristematic tissue and their role in water passage

Abstract Background Cauliflower ( Brassica oleracea L. var. botrytis ) inflorescences are composed mainly of meristematic tissue, which has a high cellular proliferation. This considerable density makes the inflorescence an organ with large proportion membranes. However, little is known about specific role lipid and protein composition plasma membrane present in this organ. Results In work, we analyzed lipids proteins from two different stages development cauliflower compared them leaf membrane. For purpose, vesicles were obtained by centrifugation for each sample vesicular diameter osmotic permeability Pf dynamic light scattering stopped-flow technique, respectively. addition, fatty acids sterols gas chromatography HPLC. The leaves was characterized HPLC-ESI-QTOF-MS data Brassicaceae UniProt database relation to presence aquaporins determined western blot analysis. highest value found 90 day inflorescences-derived (61.4 ± 4.14 ?ms ? 1 ). acids, concentrations varied according origin. profile revealed PIP1 PIP2 subfamilies both leaves. Conclusion study shows that sterols, degree unsaturation membranes give functionality water passage. represents important addition limited information available field.